About Real-Time PCR Assays

Real-time Polymerase Chain Reaction (PCR) is the ability to monitor the progress of the PCR as it occurs (i.e., in real time). Data is therefore collected throughout the PCR process, rather than at the end of the PCR. This completely revolutionizes the way one approaches PCR-based quantitation of DNA and RNA. In real-time PCR, reactions are characterized by the point in time during cycling when amplification of a target is first detected rather than the amount of target accumulated after a fixed number of cycles. The higher the starting copy number of the nucleic acid target, the sooner a significant increase in fluorescence is observed. In contrast, an endpoint assay (also called a “plate read assay”) measures the amount of accumulated PCR product at the end of the PCR cycle.

We offer both absolute and relative Quantitation assays as well as Taqman and SYBR chemistry options in order to accomodate your research needs.

Real-Time PCR Services

Real-time PCR remains one of the most sensitive tools for quantitation of nucleic acids used today. The Genomics Core offers both custom and pre-optimized assays for an assortment of applications. We offer support in all aspects of the project from assay design to data analysis. It’s our extreme attention to detail from sample QC to statistical analysis that sets our service apart from the others.

The Real-Time PCR service is advantageous to researchers conducting the following studies:

Service Features and Options