About Sequence Detection Chemistries Overview Applied Biosystems has developed two types of chemistries used to detect PCR products using Sequence Detection Systems (SDS) instruments: TaqMan® Chemistry The TaqMan chemistry uses a fluorogenic probe to enable the detection of a specific PCR product as it accumulates during PCR cycles. Assay Types that Use TaqMan Chemistry The TaqMan chemistry can be used for the following assay types:
SYBR Green I Dye Chemistry The SYBR Green I dye chemistry uses SYBR Green I dye, a highly specific, double-stranded DNA binding dye, to detect PCR product as it accumulates during PCR cycles. The most important difference between the TaqMan and SYBR Green I dye chemistries is that the SYBR Green I dye chemistry will detect all double-stranded DNA, including non-specific reaction products. A well-optimized reaction is therefore essential for accurate results. Assay Types that Use SYBR Green I Dye Chemistry The SYBR Green I dye chemistry can be used for the following assay types:
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