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Facility Services |
Labeling, Hybridization, & Data Acquisition
- Initiation of work begins when the investigator fills out a “Request
For Service Form”.
- Once the “Request for Service Form” is completed and returned
to the Molecular Genomics Core (MRB 6.158) with his/her samples,
he/she will be logged in and given a MGC (Molecular Genomics Core)
number. The request is put in the queue and is completed
in sequential number.
- The investigator has the option of designing a custom array to be hybridized or to utilize an existing array in stock. This process is described under custom array fabrication.
- The Facility will clean up the total RNA provided by passing
it through a Chroma 100 Column (CloneTech, Catalog # K1332-2). This process takes 1-2 days to complete. Although there are many methods to isolate RNA, the Molecular Genomics Core strongly recommends that you use the RNAqueous kit by Ambion,
(Catalog#1911) or Totally RNA by Ambion.
- Generation of Cy-labeled cDNA suitable for use in a Microarray
hybridization takes 2-3 days.
- Upon completion of the hybridization, washing and scanning of
the array, an analysis is performed which generates GenePix Results
(*.gpr) file.Click here for description of
files. The resulting file can be opened in Microsoft Excel
or directly imported into Spotfire for Functional Genomics via
the GenePix import tool.
- The user will receive a copy of the *.gpr in text format for
every array. Click here for
instructions on accessing your text files.
- If a user wishes to have a copy of the scanned images in either
JPEG or TIFF format, he/she will need to bring a blank CD with
his/her request form.
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