Istvan Boldogh, D.M&B., Ph.D.
Professor, Department of Microbiology and Immunology
Oxidative stress is due to reactive oxygen species (ROS), which are generated endogenously and continuously induced by environmental pollutants. Intracellularly, ROS is generated by activated intracellular oxidases due to inflammation, infections and drug treatment. Although a variety of cellular processes have evolved to eliminate ROS, oxidative stress occurs when there is a serious imbalance between production of ROS and antioxidant defense. Mitochondria are the major source of ROS and are present in all eukaroytic cells, from hundreds to thousands performing multiple cellular functions and are the major source of cellular energy. In mitochondria, ROS are formed by the univalent reduction of molecular oxygen that is mediated by respiratory complexes and via reactive compounds such as semi-ubiquinone. The long-term goal of Boldogh’s laboratory is to use multidisciplinary approaches to understand the basic mechanisms by which mitochondrial ROS is generated and are etiological agents in aging, age-related diseases and as well as augment antigen-induced allergic inflammations. Dr. Boldogh is the director of The Cell Biology Core Laboratory (CBCL) at the National Institute of Environmental Health and Sciences (NIEHS) Center at UTMB. The CBCL is dedicated to the development and delivery of state-of-the-art methods and technologies in cell biology to assist NIEHS Center Investigators in their mission. A wide range of diseases is included in the Center investigators’ research programs, such as cancer, drug abuse, behavioral disorders, chronic inflammatory lung diseases, aging and age-associated diseases. Related research ranges from studies of molecules (damaged DNA, DNA repair proteins, transcription factors, drug metabolism and interactions) and cells to those of whole animals and human populations. In addition to state-of-the-art equipment and methods, the key personnel of this core provide scientific and technical expertise that greatly benefits Center investigators in terms of experimental design, execution and interpretation.
Boldogh, I., BK. Choudhury, TK. Hazra, R. Alam, R. Goldblum, S. Mitra and S. Sur. Oxidative stress generated by pollen NADPH oxidases provide a second signal that augments antigen-induced allergic inflammation. J. Clinical Investigations, 115:2169-2179, 2005.
Bacsi, A., Choudhury, B.K., Dharajiya, N., Sur, S., and Boldogh, I. Impact of pollen-mediated oxidative stress on hypersensitivity reactions and lat-phase inflammation in allergic conjunctivitis. J. Allergy & Clinical Immunology, 116:836-43, 2005.
Choudhary S, Boldogh I, Garofalo R, Jamaluddin M, Brasier AR. Respiratory syncytial virus influences NF-kappaB-dependent gene expression through a novel pathway involving MAP3K14/NIK expression and nuclear complex formation with NF-kappaB2. Journal of Virology 79:8948-8959, 2005.
Das, A., Boldogh, I., Hazra, T., Bhakat K., and Mitra, S. Induction of the human oxidized base-specific DNA glycosylase NEIL1 by reactive oxygen species. J Biol Chem. 280(42):35272-35280, 2005.
Bacsi, A. Kannan, S. Myung-Soog Lee Hazra, TK., and Boldogh, I. Modulation of Kinase Activity of DNA-PK in Chlorambucil-Treated Cells. Free Rad Biol. Med. 39:1650–1659, 2005.
Ray, S., I. Boldogh, and A.R., Brasier. STAT3 NH2-terminal acetylation is activated by the hepatic acute-phase response and required for IL-6 induction of angiotensinogen. Gastroenterology, 129:1616-1632, 2005.
, BK., Mokkapati, SK., Boldogh, I., Hazra, TK., and Mitra, S. Acetylation of human 8-oxoguanine-DNA glycosylases by p300 and its role in 8-oxoxguanine repair in vivo. Mol. Cell Biol., 26: 1654-1665, 2006.
Das, G., Bacsi, A., M. Srivastaw, Hazra, TK. and Boldogh, I. Enhanced ?-glutamylcysteine synthetase activity ameliorates drug-induced oxidative stress levels and cytotoxicity. Molecular Carcinogenesis, 45:635-647, 2006.
Chattopadhyay R, Wiederhold L, Szczesny B, Boldogh I, Hazra TK, Izumi T, Mitra S. Identification and characterization of mitochondrial abasic (AP)-endonuclease in mammalian cells. Nucleic Acids Res. 34:2067-2076, 2006.
Forbus, J., H. Spratt, J. Wiktorowicz, Z. Wu, I. Boldogh, L. Denner, A. Kurosky, R.C. Brasier, B. Luxon and A.R. Brasier. Functional analysis of the nuclear proteome of human A549 alveolar epithelial cells by HPLC-high resolution 2D gel electrophoresis. Proteomics, 6:2656-2672, 2006.
Kruzel, ML., A. Bacsi, B. Choudhury, S. Sur, and I. Boldogh. Lactoferrin decreases pollen antigen-induced allergic airway inflammation in a murine model of asthma. Immunology 119:159-166, 2006.
Bacsi, A., M. Woodberry, W. Widger, J. J.W. Peterson, Papaconstantinou and I. Boldogh, Localization of Superoxide Anion Production to Mitochondrial Electron Transport Chain in 3-NPA-Treated Cells. Mitochondrion, 6:235–244, 2006.
Bacsi, A., B.K. Choudhury, N. Dharajiya, S. Sur, I. Boldogh, Sub-pollen particles: carriers of allergens and oxidases. J. Allergy & Clinical Immunology 118:844-850, 2006.